Combining advanced fluorescence methods and nanoagents
In this project, we will use single molecule methods to investigate the functionality of nanoagents. We will apply different advanced fluorescence techniques to follow the dynamics of proteins involved in epithelial to mesenchymal transition (Engelke B11), determine the stability of nanoagents in living cells (Wagner B4), investigate how nanoagents affect the cytoskeleton of cells (Zahler B8) or follow transported cargo in live cells and organisms. We will also use nanoagents to develope new func-tionalities for single-molecule experiments. DNA origami (Liedl A6) and metallic bow-ties or rods (Lohmüller A8) will make it possible to perform single-molecule measurements in the plasma mem-brane of cells or trap viruses.