Joint CeNS Colloquium with SFB1032
Prof. Fernando Stefani, University of Buenos Aires, Argentina
12.09.2022 at 14:00
Title: Fluorescence nanoscopy with sub-10 nm resolution
Location: Faculty of Physics, Kleiner Physikhörsaal N020 and online via Zoom
Host: Prof. Emiliano Cortés, Prof. Jochen Feldmann, Prof. Philip Tinnefeld
Summary:
Super-resolution fluorescence microscopy, also known as fluorescence nanoscopy, represented a breakthrough for bioimaging as it delivers sub-diffraction resolution using far-field microscopes. Although they do not face any fundamental limit, the resolution of the first generation of methods was bound by the limited photostability of fluorophores under ambient conditions to about 10-30 nm resolution. This has motivated the development of a second generation of fluorescence nanoscopy methods that aim to surpass sub-10 nm resolution, thus providing true molecular resolution. In this talk, I will present latest efforts of our lab to address this challenge trough three different approaches: SIMPLER1, STED-FRET2, and RASTMIN3.
(1) Szalai et al. Three-Dimensional Total-Internal Reflection Fluorescence Nanoscopy with Nanometric Axial Resolution by Photometric Localization of Single Molecules. Nat. Commun. 2021, 12 (1), 517. doi.org/10.1038/s41467-020-20863-0.
(2) Szalai et al. Super-Resolution Imaging of Energy Transfer by Intensity-Based STED-FRET. Nano Lett. 2021, 21 (5), 2296-2303. doi.org/10.1021/acs.nanolett.1c00158.
(3) Masullo et al. An Alternative to MINFLUX That Enables Nanometer Resolution in a Confocal Microscope. Light Sci. Appl. 2022, 11 (1), 199. doi.org/10.1038/s41377-022-00896-4.
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